Recently, the team of Yibing Ji and Ruijun Li from the School of Science published the research results in the internationally renowned journal Biosensor and Bioelectronics (IF: 12.6): A promising tool for clinical diagnostics: Dual-emissive carbonized polymer dots based cross-linking enhanced emission for sensitive detection of alkaline phosphatase and butyrylcholinesterase. The first author is Tingting Li, a Class of 2020 Master’s candidate. Professor Yibing Ji, Associate Professor Li Ruijun from CPU and Doctor Xu Fu of the Second Affiliated Hospital of Lanzhou University are the co-corresponding authors of this paper. China Pharmaceutical University is the first corresponding unit.
The occurrence and development of diseases in organisms affect the activity level of enzymes, that is, abnormal enzyme activity levels can predict the occurrence of diseases. Based on the principle of cross-linked enhanced emission, the research group designed dual-emission carbonized polymer dots (CPDs) with blue/red light emission. The recognition unit was constructed by the specific catalytic reaction of alkaline phosphatase (ALP) and butyrylcholinesterase (BChE), and the signal transduction unit of the ratiometric fluorescence sensor was assembled by using the specific response of CPDs to Cu2+ and the differential affinity of Cu2+ to ALP and BChE substrates/products, and the ratio sensors CPDs/Cu2+/PPi and CPDs/Cu2+/BTCh were constructed for the detection of double enzyme activity. It has been successfully used to detect ALP and BChE activity in serum samples with normal and abnormal clinical liver function, providing a new idea for the design of biosensors for rapid diagnosis of clinical diseases.
This research was supported by the National Natural Science Foundation of China Youth Program (No. 21804141) Funding.
Citations
https://doi.org/10.1016/j.bios.2023.115576
Schematic diagram of the synthesis of dual-emission CPDs and the detection of ALP and BChE activity levels